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研究報告

更新日期: 2015-02-09
點閱數: 1,576

小丑魚育種及分子標記技術之開發

年度 2014
計劃名稱 小丑魚育種及分子標記技術之開發
中文摘要 小丑魚為重要的海水觀賞魚種,因為具有鮮豔的體色及形態,因此小丑魚受歡迎的程度日趨增加。然而目前小丑魚的主要來源以野生捕捉為主,對野生族群造成威脅,為了減少自然資源的耗竭,減少人為捕撈及開發小丑魚的人工繁養殖技術已經成為最重要的課題。目前小丑魚的繁養殖關鍵技術在於生產過程(產卵、胚胎發育、孵化及訓餌),有部份小丑魚的繁養殖技術已漸趨成熟,但仍有待加強。為了區分小丑魚,需要建立一種鑑定方法,本計劃擬利用粒線體DNA分析,建立DNA密碼資料庫,提供區別小丑魚的鑑定方法。我們已完成收集五種台灣不同品系之小丑魚,也利用PCR及DNA定序分析小丑魚粒線體16S rDNA,並以MEGA軟體Test Maximum Likihood Tree分析。我們也已建立了公子小丑微衛星DNA資料庫,MS07微衛星DNA可以當作親緣鑑定之基因座。
英文摘要 The clownfish is one of the most important marine ornamental fish and is becoming increasingly popular in the aquarium fish. The main source of the clownfish is capture from the natural sources. Therefore the resource of clownfish is gradually decreased. In order to reduce the exhaustion of the natural sources, reducing capture and developing the technologies for the farming of clownfish have already become a most important subject. Currently, the development of an ornamental aquaculture protocol faces several critical bottlenecks related to production processes (i.e. spawning induction, embryo development, hatching and transition from endogenous to exogenous feeding). In order to distinguish the clownfish species, we will analyze mitochondrial DNA and construct a DNA barcode database to provide a new species identification tool for the clownfish. We have finished collect five kinds of Taiwan clownfish. We also established the clownfish (Amphiprion ocellaris) microsatellite DNA library. Microsatellite DNA MS07 can be used as a DNA marker.
計畫編號 102農科-11.3.3-漁-F3(5)
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