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研究報告

更新日期: 2012-01-04
點閱數: 2,305

白棘三列海膽(Tripneustes gratilla)繁養殖技術開發之研究

年度 2006
計劃名稱 白棘三列海膽(Tripneustes gratilla)繁養殖技術開發之研究
中文摘要 在東港生物試驗中心進行為期四個月的水溫、光週期以及飼料β-胡蘿蔔素含量的三因子海膽體成長及生殖腺成熟試驗。水溫與光週期之間的互涉效應對於海膽體成長及生殖腺的累積影響很大。在低水溫(25 ℃)養殖的海膽較在高水溫(29 ℃)者其體成長較快。而低水溫對短光週期(10L14D)條件下的海膽體成長促進效果最高其次是中光週期(12L12D)促進最少者是養在長光週期的海膽。至於生殖腺成長受到光週期與水溫互涉作用的影響則更有趣。高水溫會促進養殖在長光週期條件下海膽生殖腺的累積達3%,而低水溫對於生長在短光週期海膽生殖腺的累積可顯著提高達9%。飼料β-胡蘿蔔素含量對海膽體成長有正面效果,而對於生殖腺呈色有顯著的影響。然而飼料中增加β-胡蘿蔔素含量對於生殖腺的累積卻毫無幫助。此外飼料β-胡蘿蔔素含量是獨立因子與水溫或光週期之間無論是體成長或生殖腺指數都沒有互涉效應。本試驗自三月至十月總共進行四次海膽孵化試驗。八腕後期變態著苗試驗則在八至十月間進行。以MGDG及DGDG刺激變賴著苗試驗發現只有極少數的幼苗長初刺棘。海膽養殖如欲體成長及生殖腺累積快速建議其飼料中添加60 ppm β-胡蘿蔔素,養殖在水溫25 ℃光週期為10L14D的條件下為最佳。
英文摘要 Water temperature, photoperiod and feed β-carotene level were the three factors in a four months lasted factorial experiment to investigate the growth and gonad development of the urchin. The interaction between the two factors, water temperature and photoperiod, were significantly influencing the growth and gonad formation of the urchin investigated. Body growth rate of the urchin exhibited better growth rate cultured at 25℃ than those at 29℃. Low water temperature (25℃) had been found to elevate the urchin body growth rate within each of the three different photoperiod treatments. Interaction between water temperature and photoperiod was observed by the differences in the enhancement of body growth rate from short photoperiod (10L14D) the most followed by the mid photoperiod group (12L12D) and the least enhanced the long photoperiod group (14L10D). Gonad somatic index within short photoperiod group was increased over 9% for those urchin cultured at low temperature than those at high temperature. Feed β-carotene was an independent factor which neither interacted against of temperature factor nor the factor of photoperiod on body growth rate and gonad color enhancement. However increased the level of β-carotene in feed had no effect on the improvement of GSI value.
The larval settlement experiment of the sea urchin Tripneustes gratilla had been conducted four times from March through October 2006. None of the larvae induced by various levels of MGDG and DGDG were able to settle on the surface of the Petri-dish used in the experiment. Only very few larval grew out spines during the metamorphosis stage.
It was concluded that a 60 ppm of β-carotene level cultured at 25℃ with a short photoperiod (10L14D) may speed up both the body growth rate and gonad deposit rate of the Tripneustes gratilla.
計畫編號 95農科-14.2.1-漁-F1(2)
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