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研究報告

更新日期: 2012-01-04
點閱數: 1,639

養殖九孔黴菌性疾病致病機制及防治方法之建立研究

年度 2006
計劃名稱 養殖九孔黴菌性疾病致病機制及防治方法之建立研究
中文摘要 由九孔之分離出低等黴菌屬之形態學和孢子特性類似Traustochytrids spp.。這類黴菌之分離在低水溫之季節比較容易分離到。在組織病理學檢查上,在九孔之肝胰腺周圍之結締組織內、腸道固有層下之結締組織內和腎臟腎小管周圍之結締組織內有大量黴菌孢子堆積在吞噬細胞中。其病變之多寡和嚴重程度和季節有關。在穿透式電子顯微鏡檢查,可看到細胞質內之黴菌孢子。由分離出之黴菌萃取DNA,選殖出1個基因片段,經核酸序列定序後在基因庫並未比對出相似之病原體。在九孔幼苗藥物處理試驗方面,先將繁殖池水以抗黴藥浸泡2天,再將受精卵放入池中。此試驗重複進行兩次,並沒有增加幼苗之存活率。由本試驗之結果顯示以抗黴藥處理九孔苗並沒有增加幼苗之存活率之效果。
英文摘要 Traustochytrids spp. was isolated from cultured adult small abalone, Haliotis diversicolor supertexta. It is more frequently to isolate this fungus from abalone in low water temperature season than that of high water season. In histopathologic study, macrophages-engulfed fungus-like spores were noted in the interstitial tissues associated with hepatopancreas, intestine and kidney. This type of lesions was prominent in abalone samples collected in low water temperature season, and this finding correspond to the isolation of fungus. In transmissible electron microscopy, fungus spore-like organisms deposited in the macrophages were evident. One DNA fragment was cloned from cultured fungus using PCR technique, however, it is unable to identify the fungus species from its sequence. Treatment of pond water using antifungal chemical was unable to increase the servility of juvenile abalone in the hatchery.
計畫編號 95農科-14.2.2-漁-F1(3)
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