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研究報告

更新日期: 2012-01-04
點閱數: 2,070

箱網養殖海鱺巴斯德菌(Photobacterium damselae subsp. piscicida)疫苗研發

年度 2006
計劃名稱 箱網養殖海鱺巴斯德菌(Photobacterium damselae subsp. piscicida)疫苗研發
中文摘要 本研究以分離自罹病海鱺之病原菌巴斯德桿菌Photobacterium damselae subsp. piscicida (strain 9304)及溶藻弧菌Vibrio alginolyticus (strain AL1)對海鱺魚進行疫苗有效性之試驗研究。巴斯德桿菌調節鐵疫苗是分別以FP、YEP及BHI液態培養基添加NaCl、O.salts、dipyridyl及glucose,混合以溶藻弧菌BHIA培養基添加NaCl、O.salts、dipyridyl及glucose,以Cellophane overlay Agar方式生產調節鐵菌體及其細胞外產物,再以福馬林處理為不活化調節鐵菌苗及其細胞外產物。試驗分三種處理組,各種疫苗以腹腔注射處理海鱺魚苗(10 ± 1 g),每組注射100尾,追加接種於第四及八週各處理疫苗混合飼料以投餵方式追加一週。海鱺魚苗經疫苗處理後分別於四、八及十二週,進行活菌液攻擊試驗。第十二週結果顯示溶藻弧菌苗各組保護效果不錯,其活存率皆為96%以上。巴斯德桿菌苗在各組保護效果分別為FP組之活存率高達96%,EP組84%及BHIA組64%。本年度試驗結果與往年比較,顯示試驗菌苗之保護效果已獲提昇。因此在疫苗研發過程中,經由不斷分離強致病力菌株及特殊培養基之研發對疫苗保護成效之提昇相當重要。
英文摘要 Studies on the efficacy of vaccines were conducted in the cobia using pathogenic Ph. damselae ssp. piscicida… ( strain 9304 ) and V. alginolylicus ( strain AL1) originally isolated from moribund cage-cultured cobia. Bacterial cells and ECPs were obtained by growing the bacteria, Phdp 9304 and Val AL1 on YFP broth (adding NaCl, O.Salts, dipyridyl and glucose) and BHIA-cellophane overlay agar (adding NaCl and dipyridyl), respectively. Bacterial cells and ECPs were inactivated by 1% and 3% formalin, respectively. All the three vaccine preparations were intraperitoneally(i.p.) injected into cobia ( 10g ) with 250 fish in each treatment. After vaccination, the fish were boostered orally for one week at week 4 and 8. Fish of each treatment were lethally challenged with respectiue bacteria. The results of 12-week vaccination trials revealed that better efficacies were obseyved in treatments with bacterial cell of V.al AL1 ( 96% survival rate), bacterial cells of Phdp 9304: FP group ( 96% survival rate) ,EP group ( 84% survival rate). The present results showed better protection than those of previous year. Therefore, the continuous isolation of more virulent bacteia and manipulation of growth media are crucial to the development of better vaccines.
計畫編號 95農科-14.2.2-漁-F1(4)
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