||Shark conservation and effective fisheries management is an important and worldwide issue. Many chondrichthyan species are listed in the World Conservation Union (IUCN) Red List and Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendices II. Ample studies had used DNA barcode technique to identify the shark species from the parts of the shark body e.g., shark fin when the morphological feature was removed. Previous studies have accumulated sufficient genetic information of chondrichthyan species in the database, which enables us to apply DNA barcode as a method to identify shark species from the shark fins landed in the harbors of Taiwan as well as from the shark fins products in the retail shops. We have identified 16 species from 173 samples collected in 3 harbors and 28 species from 170 dry fins purchased from retail stories. Totally, 33 species of shark and ray were identified, which belong to 17 genera, 12 families. According the IUCN list, 3 species are in the status of Least Concern: Rhizoprionodon acutus, Rhizoprionodon taylori, and Hemigaleus australiensis, 15 species in the status of Near Threatened: Carcharhinus falciformis, Carcharhinus brevipinna, Carcharhinus melanopterus, Carcharhinus albimarginatus, Carcharhinus limbatus, Carcharhinus amblyrhynchos, Carcharhinus sorrah, Carcharhinus dussumieri, Carcharhinus galapagensis, Carcharhinus macloti, Galeocerdo cuvier, Prionace glauca, Deania quadrispinosa, Chiloscyllium punctatum, Mobula japanica, 11 species in the status of Vulnerable: Alopias pelagicus, Alopias superciliosus, Carcharhinus longimanus, Hemipristis elongata, Stegostoma fasciatum, Sphyrna zygaena, Isurus oxyrinchus, Isurus paucus, Lamna nasus, Squalus acanthias, Rhynchobatus australiae, 2 species in the status of Endangered: Sphyrna lewini, Sphyrna mokarran, 1 species in the status of Critically Endangered: Anoxypristis cuspidate.