|| Continue the development of suitable seaweed species for aquaculture and utilization in Taiwan. In this year's project we will establish a pilot plant of Bangia Conchocelis culture system in the abandoned abalone cultur ponds; 2. establish a sucessful example of long line and pond suspension aquaculture of Grateloupia; 3. shift the original plan of Okinawa mozuku aqualcultue into Hinksia michelle pond culture; 4. Pond culture of thalloid Agardhiella subulata. Regarding the nutrient feeding mode of Bangia filamentous pond culture, we observed the lower and more fluctuating growth than that in photobioreactor under controlled temperature and consistent illumination. It is due to the changing of sun light intensities and temperature during the day. It was also noted that with the same reason the culture density never got the expected densities in culture chamber. Nutrient feeding should be applied by small increments in separated weeks rather than applied in one batch. By doing this method, not only contamination can be eliminated but the over accumulation of nitrate in produce can be prevented. Regarding the application of deep ocean water, the only needed is the low temperature of it to lower the water temperature during summer season. To obtain a high density of culture, we still have to add the recipe nutrients. There will be nothing to do with the water contents. What we need is an investment of a cooling green house to have a year round production. Regarding the Grateloupia seedlings, we have successfully developed two 1 m x 1 m nets of seedling and grew them in pond with running water without a canopy. With sufficient running water and aeration, the 1N gametophyte seedlings can grow to reach the market size of more than 20 cm long after 4 months. Offshore test of the seedling nets was not possible due to the short of budget for transportation and manpower. The growth of gametophytes stopped and started to decline while summer comes. The attachment of 2N sporophyte on ropes or other substrates were not successful due to the small crust discs they formed. However the detached seedlings can still grow and reach the market size in suspension inside the tank. The sporophytes grew continuously during summer in contrast to gametophytes, but with a slower growth rate. Nutrient enrichment enhanced the epiphytes growth and retard the growth of seedlings. The amount of seedlings produced in this period were not enough to perform the running water comparison, but the expected one 20 tons culture was reached. Regarding the brown filaments Hincksia culture, 360 L indoor photobioreactor has been successfully performed routinely. However due to the harsh environment outdoors, the expansion of culture size failed in several trials. Regarding Agardhiella’s culture in pond has also been successful. The growth of Agardhiella was much better in running water mode than stop water mode, even the stop water mode had nutrients enriched. Higher replace ratio of running water showed better growth. It was speculated that the nutrient and carbon dioxide supply in running water were the main cause of the different growth, but the best replace ratio of water for the growth of Agardhiella is not known yet. The cost for four different types of algae have been analyzed. The Bangia and Hincksia filamentous culture needed a lot of manpower in cleaning the ponds and harvesting in addition to the machines for harvesting. So the cost of filamentous culture will be 3000NT/kg dry powder, that means 500NT/kg wet filaments plus drying operation cost. The Hincksia filamentous culture will be costly, about double of the cost in dealing with Bangia, 6000NT/kg. The culture of Agardhiella and Grateloupia are still below the market size, and the mixing system is not well designed, the estimation of cost is not reasonable at this moment. If they were cultured in an open sea the cost will be the labor of tying the fragments on rope. If by tank culture, the cost will be on the replace ratio of water, the higher ratio, the higher growth rate, but more cost. Regarding the technology demonstration, we took the business matching demonstration and exhibition to the industry investors, rather than the fishermen, including the presentation of “Inland Culture of Filamentous Seaweed for the Source Material of Nutraceutical and Biomedical Products” at Kaoshiung on April 11, and the “Culture of Functional Seaweed for Food Supplement through Tissue Culture and Seedling Development” on June 6. We also participated the Taiwan Academic Research Expo 2013 to show our products and technologies. Finally we have succeeded a spin-off contract with Ray-In Biotechnology Ltd. Co. on the development of nutraceutical and pharmaceutical source material from red and brown algal aquaculture.